| 质粒名称: | Lentiviral mPer2-Luc |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 182761 |
| 存储实验室: | Qing-Jun Meng |
|
载体骨架基本信息 |
|
| 载体名称: | Lentiviral transfer vector |
| 载体出品公司: | Clontech |
| 空载体大小: | 7400bp |
| 完整质粒大小: | 7800 bp |
| 载体类型: | Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | mouse Per2 gene promoter |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 419bp |
| 启动子: | Mouse Per2 promoter |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | Nde I (not destroyed) |
| 3’克隆位点: | Sal I (not destroyed) |
| 5’测序引物 | TCTCTGCCGGCTGTGAGTTG |
| 3’测序引物 | CCGCTAGTCCCAGTAGCGC |
|
文献引用方法 |
|
| 材料和方法部分: | Lentiviral mPer2-Luc was a gift from Qing-Jun Meng (Addgene plasmid # 182761) |
| 参考文献部分: | The circadian clock in murine chondrocytes regulates genes controlling key aspects of cartilage homeostasis. Gossan N, Zeef L, Hensman J, Hughes A, Bateman JF, Rowley L, Little CB, Piggins HD, Rattray M, Boot-Handford RP, Meng QJ. Arthritis Rheum. 2013 Sep;65(9):2334-45. doi: 10.1002/art.38035. 10.1002/art.38035 PubMed 23896777 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Lentiviral mPer2-Luc 8743 bp DNA circular SYN 27-AUG-2024
DEFINITION Lentiviral packaging vector containing the promoter region of mouse
Per2 gene driving a luciferase reporter.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8743)
AUTHORS Gossan N, Zeef L, Hensman J, Hughes A, Bateman JF, Rowley L, Little
CB, Piggins HD, Rattray M, Boot-Handford RP, Meng QJ
TITLE The circadian clock in murine chondrocytes regulates genes
controlling key aspects of cartilage homeostasis.
JOURNAL Arthritis Rheum. 2013 Sep;65(9):2334-45. doi: 10.1002/art.38035.
PUBMED 23896777
REFERENCE 2 (bases 1 to 8743)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 27, 2024 from SnapGene Server 7.0.3
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1002/art";
journalName: "Arthritis Rheum"; date: "2013-09"; volume: "65";
issue: "9"; pages: "2334-45"
FEATURES Location/Qualifiers
source 1..8743
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 227..352
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 845..1078
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1263..1307
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1574..1690
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind 1892..1911
/label=RVprimer3
/note="pGL3 vector, forward primer"
regulatory 2425..2434
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2431..4083
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(2497..2515)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
polyA_signal 4124..4245
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 4359..4947
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4412..4432)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4830..4841)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 5034..5267
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5339..5460
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 5500..5635
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(5655..5674)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5656..5674)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5684..5701)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5684..5700)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5693..5715)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5842..6297
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5929..5948)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6139..6160
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6323..6427
/gene="bla"
/label=AmpR promoter
CDS 6428..7288
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6646..6665)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7459..8047
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7948..7967
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8201..8218
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8335..8356
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8371..8401
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8409..8425
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8414..8436
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8433..8449
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8433..8449
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8468..8488
/label=T3
/note="T3 promoter, forward primer"
promoter 8470..8488
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 8516..8742
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR join(8743,1..180)
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
1 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
61 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
661 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
721 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
781 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
841 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
901 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
961 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
1021 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
1081 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
1141 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
1201 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
1261 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
1321 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
1381 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
1441 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
1501 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac
1561 ggtatcggtt aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
1621 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat
1681 tcaaaatttt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg
1741 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
1801 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
1861 cacttggcag tacatcaagt gtatcatatg actagcaaaa taggctgtcc ccagtgcaag
1921 tgcaggtgcc agaacatttc tctatcgata ggtacctctg ccggctgtga gttgcgcagc
1981 ggccaagcac cattcccccg tgccgcagtg gtacgcgcca ctccggggct gcacgagcgg
2041 gccaccgccg tgccaggtga atggaagtcc cgcaggccgg aagtggacga gcctactctc
2101 ccgggcgcgg gggggcgcaa gagcgcgcag catcttcatt gaggaacccg ggcggcgaac
2161 atggagttcc atgtgcgtct tatgtaaaga gagcgacggg cgtctccacc aattgatgag
2221 cgtagctctc aggttccgcc ccgccagtat gcaaatgagg tggcactccg accaatggcg
2281 cgcgcagggg cgggctcagc gcgcgcggtc acgttttcca ctatgtgaca gcggagggcg
2341 acgcggcggc agcggcgcta ctgggactag cggtctcgag atctgcgatc taagtaagct
2401 tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc
2461 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
2521 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
2581 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
2641 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
2701 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
2761 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
2821 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
2881 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
2941 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
3001 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg
3061 cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa
3121 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
3181 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
3241 gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc
3301 ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
3361 gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg
3421 ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc agctattctg
3481 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
3541 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg
3601 tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
3661 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
3721 cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct
3781 cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca
3841 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
3901 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
3961 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
4021 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg
4081 taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag
4141 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
4201 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
4261 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
4321 ctctacaaat gtggtaaaat cgataaggat ccgtcgacaa tcaacctctg gattacaaaa
4381 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
4441 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
4501 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
4561 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
4621 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
4681 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
4741 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
4801 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
4861 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
4921 ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat
4981 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
5041 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
5101 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
5161 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
5221 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
5281 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
5341 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
5401 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
5461 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
5521 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
5581 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
5641 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
5701 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
5761 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
5821 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
5881 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
5941 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
6001 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
6061 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
6121 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
6181 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
6241 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
6301 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
6361 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
6421 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
6481 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
6541 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
6601 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
6661 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
6721 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
6781 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
6841 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
6901 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
6961 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
7021 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
7081 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
7141 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
7201 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
7261 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
7321 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
7381 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
7441 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
7501 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
7561 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
7621 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
7681 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
7741 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
7801 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
7861 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
7921 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
7981 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
8041 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
8101 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
8161 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
8221 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
8281 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
8341 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
8401 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
8461 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
8521 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
8581 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
8641 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
8701 tgcagagata ttgtatttaa gtgcctagct cgatacataa acg
//
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